pRP.ABCA4 c.5461-10T
(Plasmid
#226553)
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PurposeMinigene encoding the ABCA4 c.5461-10T mutation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRP
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Backbone manufacturerVectorBuilder
- Backbone size w/o insert (bp) 2297
- Total vector size (bp) 11196
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameABCA4
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SpeciesH. sapiens (human)
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Insert Size (bp)9144
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Mutationc.5461-10T intronic mutation
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GenBank IDNG_009073
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Entrez GeneABCA4 (a.k.a. ABC10, ABCR, ARMD2, CORD3, FFM, RMP, RP19, STGD, STGD1)
- Promoter CAG
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRP.ABCA4 c.5461-10T was a gift from Dave Wee (Addgene plasmid # 226553 ; http://n2t.net/addgene:226553 ; RRID:Addgene_226553) -
For your References section:
Unveiling Sequence-Agnostic Mixed-Chemical Modification Patterns for SpliceSwitching Oligonucleotides using the NATURA platform. Tommaso T, Agarwal T, Cher WY, Ow JR, Chew AK, Qian Sun PY, Reddy Gurrampati RS, Lu H, Naidu P, Ng HK, Le Guezennec X, Ng SY, Lakshmanan M, Ernesto G, Wee KB. Molecular Therapy: Nucleic Acid (2025) 10.1016/j.omtn.2024.102422