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pMyt1(502-623)
(Plasmid #22655)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22655 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIND
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6360
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myelin Transcription Factor 1
  • Alt name
    Myt1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    364
  • Mutation
    contains only first 1/2 of Myt1 central domain (CD) 2035-2399bp)
  • Entrez Gene
    Myt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T3 Promoter
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

First 1/2 of Myt1 central domain. bp 2035-2399 and aa 602-623. Used for Mammalian 2-hybrid assays

This Myt1 DNA cloned by Hudson lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyt1(502-623) was a gift from Lynn Hudson (Addgene plasmid # 22655 ; http://n2t.net/addgene:22655 ; RRID:Addgene_22655)

  • For your References section:

    Myt1 family recruits histone deacetylase to regulate neural transcription. Romm E, Nielsen JA, Kim JG, Hudson LD. J Neurochem. 2005 Jun . 93(6):1444-53. 10.1111/j.1471-4159.2005.03131.x PubMed 15935060
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