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Addgene

pDest17_A1-LCD swap X5
(Plasmid #226547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest17
  • Backbone manufacturer
    Invtirogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    A1-LCD swap X5
  • Species
    H. sapiens (human)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TEV cleavage Site. Growth strain BL21_DE3 also recommended for this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest17_A1-LCD swap X5 was a gift from Tanja Mittag (Addgene plasmid # 226547 ; http://n2t.net/addgene:226547 ; RRID:Addgene_226547)

  • For your References section:

    Design of intrinsically disordered protein variants with diverse structural properties. Pesce F, Bremer A, Tesei G, Hopkins JB, Grace CR, Mittag T, Lindorff-Larsen K. Sci Adv. 2024 Aug 30;10(35):eadm9926. doi: 10.1126/sciadv.adm9926. Epub 2024 Aug 28. 10.1126/sciadv.adm9926 PubMed 39196930
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