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pmSin3BSF274 (short form)
(Plasmid #22653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACT
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5565
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mSin3B
  • Alt name
    Sin3B homolog B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    821
  • Entrez Gene
    Sin3b (a.k.a. 2810430C10Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7 Promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to see if truncated form of mSin3b would be biologically active when binding Mouse Myt1

This short form of mSIN3B was PCR'd from the full-length clone (pcDNAAmSin3B-Flag) which was a gift of Dr. Ronald dePinho.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmSin3BSF274 (short form) was a gift from Lynn Hudson (Addgene plasmid # 22653 ; http://n2t.net/addgene:22653 ; RRID:Addgene_22653)

  • For your References section:

    Myt1 family recruits histone deacetylase to regulate neural transcription. Romm E, Nielsen JA, Kim JG, Hudson LD. J Neurochem. 2005 Jun . 93(6):1444-53. 10.1111/j.1471-4159.2005.03131.x PubMed 15935060
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