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pSecTaq2-hGas6-Myc-6xHis
(Plasmid #226523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSecTag2
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 5527
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human GAS6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1944
  • Entrez Gene
    GAS6 (a.k.a. AXLLG, AXSF)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer GGTTCCACTGGTGACAACTTTCTTAGTAAA
  • 3′ sequencing primer TCCTCGAGCGGCCGCAGAATTTTTCGTTTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSecTaq2-hGas6-Myc-6xHis was a gift from Raymond Birge & Sergei Kotenko (Addgene plasmid # 226523 ; http://n2t.net/addgene:226523 ; RRID:Addgene_226523)

  • For your References section:

    Requirement of Gamma-Carboxyglutamic Acid Modification and Phosphatidylserine Binding for the Activation of Tyro3, Axl, and Mertk Receptors by Growth Arrest-Specific 6. Geng K, Kumar S, Kimani SG, Kholodovych V, Kasikara C, Mizuno K, Sandiford O, Rameshwar P, Kotenko SV, Birge RB. Front Immunol. 2017 Nov 10;8:1521. doi: 10.3389/fimmu.2017.01521. eCollection 2017. 10.3389/fimmu.2017.01521 PubMed 29176978
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