pMycMyt1-7zf/IRES-Red
(Plasmid #22652)

• Depositing Lab: Lynn Hudson
• Publication: Nielsen et al Mol Cell Neurosci. 2004 Jan . 25(1):111-23.

Backbone

• Vector backbone: pIRESRed
• Backbone manufacturer: R. Armstrong
• Backbone size w/o insert (bp): 5635
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Myelin Transcription Factor 1
• Alt name: Myt1
• Species: M. musculus (mouse)
• Insert Size (bp): 3870
• Entrez Gene: Myt1 (a.k.a. Nzf2, Nzf2a, Nzf2b, Nztf2)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Hechsher-Sorensen
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mu Myt1 complete sequence includes 7 Zn Fingers and a Myc Tag. This was subcloned into pIRES/Red made by R. A. Armstrong. The DsRed does not express due to problem with IRES

This Myt1-7zf DNA was cut from the full-length clone (pMycMyt1-7zf) which was a gift of Dr. Jacob Hecksher-Sorensen.