gamma-tubulin (1-335)
(Plasmid #226519)

• Purpose: Expressing a fragment of gamma-tubulin containing gamma-tubulin GTPase domain
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii: S0167-4889(17)30283-5. doi: 10.1016/j.bbamcr.2017.10.008.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6436
• Vector type: Mammalian Expression ; pcDNA3.1
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TUBG1 N-terminal amino acids 1-335
• Alt name: gamma-tubulin 1
• Species: H. sapiens (human)
• Insert Size (bp): 1008
• Mutation: Deleted amino acids 334-451 and introduced the following point mutations: T at bp28 to C, G at bp30 to A, C at bp33 to A, and G at bp36 to A. These are silent mutations introduced to achieve single-guide RNA resistance. A stop codon was added after amino acid 335.
• GenBank ID: NM_001070.5 NM_001070.5
• Entrez Gene: TUBG1 (a.k.a. CDCBM4, GCP-1, TUBG, TUBGCP1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: T7 promoter primer
• 3′ sequencing primer: BGH Reverse Primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry