pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP
(Plasmid
#226516)
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PurposeMamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226516 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTOMM20-SNAP-tag2-T2A-EGFP
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.28.610127 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP was a gift from Kai Johnsson (Addgene plasmid # 226516 ; http://n2t.net/addgene:226516 ; RRID:Addgene_226516) -
For your References section:
SNAP-tag2: faster and brighter protein labeling. Kühn S, Nasufovic V, Wilhelm J, Kompa J, de Lange EMF, Lin Y-H, Egoldt C, Fischer J, Lennoi A, Tarnawski M, Reinstein J, Vlijm R, Hiblot J, Johnsson K. bioRxiv 2024.08.28.610127 10.1101/2024.08.28.610127