pLH116
(Plasmid #22651)

• Depositing Lab: Lynn Hudson
• Publication: Hudson et al Proc Natl Acad Sci U S A. 1987 Mar . 84(5):1454-8.

Backbone

• Vector backbone: pGEM3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2867
• Vector type: cloning vector
• Selectable markers: LacZ

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Proteolipid Protein
• Alt name: PLP
• Species: M. musculus (mouse)
• Insert Size (bp): 980
• Entrez Gene: Plp1 (a.k.a. DM20, Plp, jimpy, jp, msd, rsh)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains entire coding region of the mouse PLP cDNA including part of the 5' UTR. Used to make riboprobes by transcribing with SP6 polymerase to give a 980 bp antisense riboprobe or transcribing with T7 polymerase to give a 980bp sense riboprobe for RNA analysis on Northern blots.

This PLP DNA cloned by Hudson lab.