pCMV-iGlu3Fast
(Plasmid #226500)

• Purpose: Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release
• Depositing Lab: Katalin Torok
• Publication: Tran et al BioRxiv, August 30th, 2024

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3924
• Total vector size (bp): 5778
• Modifications to backbone: EGFP has been removed by restriction enzymes
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: iGluSnFR3 S72T
• Alt name: iGlu3Fast
• Species: Synthetic
• Insert Size (bp): 1854
• Mutation: S72T
• Promoter: CMV
• Tags / Fusion Proteins:
  • IgK (N terminal on insert)
  • Myc tag (C terminal on insert)
  • PDGFR Beta TM domain (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SV40 pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The iGluSnFR3 insert came from pAAV iGluSnFR3 (Addgene 178329), we cloned into the pEGFP-N1 vector and introduced the S72T mutation

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry