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pR-Avr
(Plasmid #22650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-3Z
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2743
  • Vector type
    Cloning and RNA Synthesis
  • Selectable markers
    LacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myelin Transcription Factor 1
  • Alt name
    Myt1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    700
  • Entrez Gene
    Myt1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer SP6 Promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used to make riboprobes by cutting with SmaI and transcribing with SP6 polymerase to give a 700 bp antisense riboprobe or HindIII and transcribing with T7 polymerase to give a 700bp sense riboprobe for RNA analysis on Northern blots. For 3' UTR of cDNA

This Rat Myt1 DNA cloned by Hudson lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR-Avr was a gift from Lynn Hudson (Addgene plasmid # 22650 ; http://n2t.net/addgene:22650 ; RRID:Addgene_22650)

  • For your References section:

    Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD. J Neurosci Res. 1997 Oct 15. 50(2):272-90. 10.1002/(SICI)1097-4547(19971015)50:2<272::AID-JNR16>3.0.CO;2-A PubMed 9373037
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