pR-Pst
(Plasmid
#22648)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-3Z
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2743
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Vector typeCloning and RNA Synthesis
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Selectable markersLacZ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMyelin Transcription Factor 1
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Alt nameMyt1
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1500
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Entrez GeneMyt1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer SP6 Promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Used to make Sense (HindIII) and antisense probes (XbaI) (1.5kb) for RNA analysis on Northern blots
This Rat Myt1 DNA cloned by Hudson lab.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pR-Pst was a gift from Lynn Hudson (Addgene plasmid # 22648 ; http://n2t.net/addgene:22648 ; RRID:Addgene_22648) -
For your References section:
Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system. Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD. J Neurosci Res. 1997 Oct 15. 50(2):272-90. 10.1002/(SICI)1097-4547(19971015)50:2<272::AID-JNR16>3.0.CO;2-A PubMed 9373037