pCDNA3 HaloTag-V5-TurboID
(Plasmid
#226474)
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PurposeExpresses fusion of HaloTag and TurboID in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTurboID
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GenBank ID
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Tags
/ Fusion Proteins
- V5 (N terminal on insert)
- HaloTag (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3 HaloTag-V5-TurboID was a gift from Hyun-Woo Rhee (Addgene plasmid # 226474 ; http://n2t.net/addgene:226474 ; RRID:Addgene_226474) -
For your References section:
Identification of proteomic landscape of drug-binding proteins in live cells by proximity-dependent target ID. Kwak C, Park C, Ko M, Im CY, Moon H, Park YH, Kim SY, Lee S, Kang MG, Kwon HJ, Hong E, Seo JK, Rhee HW. Cell Chem Biol. 2022 Dec 15;29(12):1739-1753.e6. doi: 10.1016/j.chembiol.2022.10.001. Epub 2022 Oct 21. 10.1016/j.chembiol.2022.10.001 PubMed 36272407