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pJB008
(Plasmid #22647)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTZ19R
  • Backbone manufacturer
    Fermentas
  • Backbone size w/o insert (bp) 2862
  • Vector type
    phagemid
  • Selectable markers
    LacZ

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human genomic PLP
  • Alt name
    Human Proteolipid Protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    10000
  • Entrez Gene
    PLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13 PUC
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genomic DNA from an EMBL3 human genomic library. Contains 3' end of Exon1, Intron 1, Exon 2 and Intron 2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB008 was a gift from Lynn Hudson (Addgene plasmid # 22647 ; http://n2t.net/addgene:22647 ; RRID:Addgene_22647)

  • For your References section:

    Mutation of the proteolipid protein gene PLP in a human X chromosome-linked myelin disorder. Hudson LD, Puckett C, Berndt J, Chan J, Gencic S. Proc Natl Acad Sci U S A. 1989 Oct . 86(20):8128-31. 10.1073/pnas.86.20.8128 PubMed 2479017
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