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RLuc_MET_95_FLuc_Pless
(Plasmid #226464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Predicted MET IRES

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (destroyed during cloning)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer Amp-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RLuc_MET_95_FLuc_Pless was a gift from Paolo Comoglio (Addgene plasmid # 226464 ; http://n2t.net/addgene:226464 ; RRID:Addgene_226464)

  • For your References section:

    The integrated stress response drives MET oncogene overexpression in cancers. Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM. EMBO J. 7 Jan 2025. doi: 10.1038/s44318-024-00338-4. 10.1038/s44318-024-00338-4
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