pGL4.23[uORF2/luc2/minP]
(Plasmid
#226460)
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PurposeMET 5'UTR (uORF2 mutated) upstream FLuc
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226460 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMET 5'UTR uORF2 mutated
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pGL3FOR (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.23[uORF2/luc2/minP] was a gift from Paolo Comoglio (Addgene plasmid # 226460 ; http://n2t.net/addgene:226460 ; RRID:Addgene_226460) -
For your References section:
The integrated stress response drives MET oncogene overexpression in cancers. Cerqua M, Foiani M, Boccaccio C, Comoglio PM, Altintas DM. EMBO J. 7 Jan 2025. doi: 10.1038/s44318-024-00338-4. 10.1038/s44318-024-00338-4