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Addgene

pMyt1-d
(Plasmid #22645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myelin Transcription Factor 1: Myt1 (aa 493-1002) (bp 2009 to 3557))
  • Alt name
    Myt1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1549
  • Entrez Gene
    MYT1 (a.k.a. MTF1, MYTI, PLPB1, C20orf36)
  • Tag / Fusion Protein
    • T7-TAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglI (destroyed during cloning)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 terminator primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

622 aa fusion protein containing Myt1 aa 493 to 1002 (bp 2009 to 3557). Includes downstream cluster of 4 Zn fingers and flanking regions. Used to generate anti-Myt1-D antibody. ALIAS: pBgl-9

This Myt1 DNA cloned by Hudson lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyt1-d was a gift from Lynn Hudson (Addgene plasmid # 22645 ; http://n2t.net/addgene:22645 ; RRID:Addgene_22645)

  • For your References section:

    Novel member of the zinc finger superfamily: A C2-HC finger that recognizes a glia-specific gene. Kim JG, Hudson LD. Mol Cell Biol. 1992 Dec . 12(12):5632-9. PubMed 1280325
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