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Addgene

TINGL-RR pDRF1
(Plasmid #226435)

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Item Catalog # Description Quantity Price (USD)
Plasmid 226435 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDRF1-GW
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TINGL-O2A-ymScarletI
  • Alt name
    TINGL-RR
  • Alt name
    mTq2-based glucose sensor-O2A-ymScarletI
  • Species
    S. cerevisiae (budding yeast)
  • Promoter PMA1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer AACAATCGTTAATAATTAATTAATTGG
  • 3′ sequencing primer GAGTCACTTTAAAATTTGTATACAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.29.626064 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TINGL-RR pDRF1 was a gift from Bas Teusink (Addgene plasmid # 226435 ; http://n2t.net/addgene:226435 ; RRID:Addgene_226435)

  • For your References section:

    An mTurquoise2-based glucose biosensor. Botman D, Tielman A, Goedhart J, Teusink B. bioRxiv 2024.11.29.626064 10.1101/2024.11.29.626064
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