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Addgene

pCMV-EGFP-nonstop
(Plasmid #226406)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226406 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clonetech
  • Total vector size (bp) 4601
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP without stop codon
  • Insert Size (bp)
    717
  • GenBank ID
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.13.584747 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-EGFP-nonstop was a gift from Nico Dantuma (Addgene plasmid # 226406 ; http://n2t.net/addgene:226406 ; RRID:Addgene_226406)
  • For your References section:

    Chemical inhibition of the integrated stress response impairs the ubiquitin-proteasome system. Xu S, Gierisch ME, Barchi E, Poser I, Alberti S, Salomons FA, Dantuma NP. Commun Biol. 2024 Oct 8;7(1):1282. doi: 10.1038/s42003-024-06974-0. 10.1038/s42003-024-06974-0 PubMed 39379572