pCMV-EGFP-nonstop
(Plasmid
#226406)
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PurposeExpresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226406 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClonetech
- Total vector size (bp) 4601
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP without stop codon
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Insert Size (bp)717
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GenBank ID
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV FW
- 3′ sequencing primer SV40 RS (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.13.584747 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-EGFP-nonstop was a gift from Nico Dantuma (Addgene plasmid # 226406 ; http://n2t.net/addgene:226406 ; RRID:Addgene_226406) -
For your References section:
Chemical inhibition of the integrated stress response impairs the ubiquitin-proteasome system. Xu S, Gierisch ME, Barchi E, Poser I, Alberti S, Salomons FA, Dantuma NP. Commun Biol. 2024 Oct 8;7(1):1282. doi: 10.1038/s42003-024-06974-0. 10.1038/s42003-024-06974-0 PubMed 39379572