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pET-22b(+) pCopA RBS30 sfgfp
(Plasmid #226371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-22b(+)
  • Backbone size w/o insert (bp) 5539
  • Total vector size (bp) 6559
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Species
    E.coli
  • Promoter copA promoter (pCopA) and RBS30 regulation
  • Tag / Fusion Protein
    • 6x HisTag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-22b(+) pCopA RBS30 sfgfp was a gift from Urartu Seker (Addgene plasmid # 226371 ; http://n2t.net/addgene:226371 ; RRID:Addgene_226371)

  • For your References section:

    Genetically engineered bacterial biofilm materials enhances portable whole cell sensing. Koksaldi IC, Avci E, Kose S, Ozkul G, Kehribar ES, Safak Seker UO. Biosens Bioelectron. 2024 Aug 10;264:116644. doi: 10.1016/j.bios.2024.116644. 10.1016/j.bios.2024.116644 PubMed 39137519
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