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Addgene

pAM420 GFP-titin-ssrA substrate
(Plasmid #226362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-titin substrate
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM420 GFP-titin-ssrA substrate was a gift from Andreas Martin (Addgene plasmid # 226362 ; http://n2t.net/addgene:226362 ; RRID:Addgene_226362)

  • For your References section:

    Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome. Bashore C, Dambacher CM, Goodall EA, Matyskiela ME, Lander GC, Martin A. Nat Struct Mol Biol. 2015 Sep;22(9):712-9. doi: 10.1038/nsmb.3075. Epub 2015 Aug 24. 10.1038/nsmb.3075 PubMed 26301997
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