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Addgene

pAM239 Rpn10
(Plasmid #226357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rpn10
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPN10 (a.k.a. YHR200W, MCB1, SUN1)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM239 Rpn10 was a gift from Andreas Martin (Addgene plasmid # 226357 ; http://n2t.net/addgene:226357 ; RRID:Addgene_226357)

  • For your References section:

    Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase. Beckwith R, Estrin E, Worden EJ, Martin A. Nat Struct Mol Biol. 2013 Oct;20(10):1164-72. doi: 10.1038/nsmb.2659. Epub 2013 Sep 8. 10.1038/nsmb.2659 PubMed 24013205
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