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pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1
(Plasmid #226353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    titin
  • Species
    H. sapiens (human)
  • Mutation
    V15P
  • Entrez Gene
    TTN (a.k.a. CMD1G, CMH9, CMPD4, CMYO5, CMYP5, EOMFC, HMERF, LGMD2J, LGMDR10, MYLK5, SALMY, TMD)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1 was a gift from Andreas Martin (Addgene plasmid # 226353 ; http://n2t.net/addgene:226353 ; RRID:Addgene_226353)

  • For your References section:

    Ubiquitin modulates 26S proteasome conformational dynamics and promotes substrate degradation. Jonsson E, Htet ZM, Bard JAM, Dong KC, Martin A. Sci Adv. 2022 Dec 23;8(51):eadd9520. doi: 10.1126/sciadv.add9520. Epub 2022 Dec 23. 10.1126/sciadv.add9520 PubMed 36563145
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