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pEF1a-mRor2Y641F
(Plasmid #22635)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22635 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a-myc/his A
  • Backbone size w/o insert (bp) 9226
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ror2 Y641F
  • Alt name
    Ror2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2835
  • Mutation
    Changed Tyrosine 641 to Phenylalanine
  • GenBank ID
    AB010384
  • Entrez Gene
    Ror2 (a.k.a. Ntrkr2)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Y. Minami (Japan)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-mRor2Y641F was a gift from Roel Nusse (Addgene plasmid # 22635 ; http://n2t.net/addgene:22635 ; RRID:Addgene_22635)

  • For your References section:

    The Ror2 receptor requires tyrosine kinase activity to mediate Wnt5a signaling. Mikels A, Minami Y, Nusse R. J Biol Chem. 2009 Aug 31. ():. 10.1074/jbc.M109.041715 PubMed 19720827
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