pAM315 pACYC-His6-Rpn10[ΔUIM]
(Plasmid
#226348)
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PurposeExpression of yeast Rpn10 with the UIM deleted
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerpn10
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SpeciesS. cerevisiae (budding yeast)
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Mutationdelta UIM
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Entrez GeneRPN10 (a.k.a. YHR200W, MCB1, SUN1)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAM315 pACYC-His6-Rpn10[ΔUIM] was a gift from Andreas Martin (Addgene plasmid # 226348 ; http://n2t.net/addgene:226348 ; RRID:Addgene_226348) -
For your References section:
Ubiquitin modulates 26S proteasome conformational dynamics and promotes substrate degradation. Jonsson E, Htet ZM, Bard JAM, Dong KC, Martin A. Sci Adv. 2022 Dec 23;8(51):eadd9520. doi: 10.1126/sciadv.add9520. Epub 2022 Dec 23. 10.1126/sciadv.add9520 PubMed 36563145