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Addgene

pRSET A-mEosEM-E
(Plasmid #226336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETa
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEosEM-E
  • Species
    Synthetic
  • Insert Size (bp)
    681
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET A-mEosEM-E was a gift from Pingyong Xu (Addgene plasmid # 226336 ; http://n2t.net/addgene:226336 ; RRID:Addgene_226336)

  • For your References section:

    Improved Fluorescent Proteins for Dual-Colour Post-Embedding CLEM. Peng D, Li N, He W, Drasbek KR, Xu T, Zhang M, Xu P. Cells. 2022 Mar 23;11(7):1077. doi: 10.3390/cells11071077. 10.3390/cells11071077 PubMed 35406640
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