pBz12
(Plasmid
#226321)
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PurposeE. coli protein expression for protein purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 226321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET14b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6xHis-Rubisco
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SpeciesH. neapolitanus
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Insert Size (bp)1443
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBz12 was a gift from David Savage (Addgene plasmid # 226321 ; http://n2t.net/addgene:226321 ; RRID:Addgene_226321) -
For your References section:
Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly. Turnsek JB, Oltrogge LM, Savage DF. J Biol Chem. 2024 Jul 4;300(8):107532. doi: 10.1016/j.jbc.2024.107532. 10.1016/j.jbc.2024.107532 PubMed 38971311