Skip to main content
Addgene

pJB65
(Plasmid #226319)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226319 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET14b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    6xHis- (VTG to AAA MR1-6)-strep
  • Species
    H. neapolitanus
  • Insert Size (bp)
    1092
  • Mutation
    N-terminal domain deleted, C-terminal domain deleted, VTG24-26AAA, VTG35-37AAA, VSG46-48AAA, VSG83-85AAA, VTG94-96AAA, VTG105-107AAA, VSG144-146AAA, VTG155-157AAA, VTG199-201AAA, VTG210-2AAA, VTG258-260AAA, VTG269-271AAA, VTG280-282AAA
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • Strep (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB65 was a gift from David Savage (Addgene plasmid # 226319 ; http://n2t.net/addgene:226319 ; RRID:Addgene_226319)

  • For your References section:

    Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly. Turnsek JB, Oltrogge LM, Savage DF. J Biol Chem. 2024 Jul 4;300(8):107532. doi: 10.1016/j.jbc.2024.107532. 10.1016/j.jbc.2024.107532 PubMed 38971311
Related items: