pEF1a-mRor2N620K
(Plasmid #22631)

• Depositing Lab: Roel Nusse
• Publication: Mikels et al J Biol Chem. 2009 Aug 31. ():.

Backbone

• Vector backbone: pEF1a-myc/his A
• Backbone size w/o insert (bp): 9226
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ror2 N620K
• Alt name: Ror2
• Species: M. musculus (mouse)
• Insert Size (bp): 2835
• Mutation: Changed Asparagine 620 to Lysine
• GenBank ID: AB010384
• Entrez Gene: Ror2 (a.k.a. Ntrkr2)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Y. Minami (Japan)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry