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Addgene

pJB53
(Plasmid #226309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSAV108
  • Backbone manufacturer
    Savage Lab
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-3
  • Species
    H. neapolitanus
  • Insert Size (bp)
    2613
  • Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AAA, VTG343-5AAA, VTG354-6AAA, VSG393-5AAA, VTG404-6AAA,
  • Promoter pTRC

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB53 was a gift from David Savage (Addgene plasmid # 226309 ; http://n2t.net/addgene:226309 ; RRID:Addgene_226309)

  • For your References section:

    Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly. Turnsek JB, Oltrogge LM, Savage DF. J Biol Chem. 2024 Jul 4;300(8):107532. doi: 10.1016/j.jbc.2024.107532. 10.1016/j.jbc.2024.107532 PubMed 38971311
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