SBC015910
(Plasmid
#226287)
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PurposeExpresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226287 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbB1k
- Total vector size (bp) 6478
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameConiferyl alcohol acyltransferase
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Alt nameCFAT
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SpeciesPetunia hybrida
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Insert Size (bp)1365
Gene/Insert 2
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Gene/Insert nameIsoeugenol synthase 1
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Alt nameIGS1
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SpeciesPetunia hybrida
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Insert Size (bp)972
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UniProt IDs:
CFAT - A1XWY7
IGS1 - Q15GI3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SBC015910 was a gift from Rainer Breitling (Addgene plasmid # 226287 ; http://n2t.net/addgene:226287 ; RRID:Addgene_226287) -
For your References section:
Bioproduction of methylated phenylpropenes and isoeugenol in Escherichia coli. Chua J, Hanko EKR, Yiakoumetti A, Stoney RA, Chromy J, Valdehuesa KNG, Hollywood KA, Yan C, Takano E, Breitling R. Metab Eng Commun. 2024 May 15;18:e00237. doi: 10.1016/j.mec.2024.e00237. eCollection 2024 Jun. 10.1016/j.mec.2024.e00237 PubMed 38799229