SBC009876
(Plasmid
#226283)
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PurposeExpresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbB1k
- Total vector size (bp) 6492
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameConiferyl alcohol acyltransferase
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Alt nameCFAT
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SpeciesPetunia hybrida
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Insert Size (bp)1365
Gene/Insert 2
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Gene/Insert nameEugenol synthase 1
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Alt nameEGS1
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SpeciesOcimum basilicum
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Insert Size (bp)945
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SBC009876 was a gift from Nigel Scrutton (Addgene plasmid # 226283 ; http://n2t.net/addgene:226283 ; RRID:Addgene_226283) -
For your References section:
Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers. Robinson CJ, Carbonell P, Jervis AJ, Yan C, Hollywood KA, Dunstan MS, Currin A, Swainston N, Spiess R, Taylor S, Mulherin P, Parker S, Rowe W, Matthews NE, Malone KJ, Le Feuvre R, Shapira P, Barran P, Turner NJ, Micklefield J, Breitling R, Takano E, Scrutton NS. Metab Eng. 2020 Jul;60:168-182. doi: 10.1016/j.ymben.2020.04.008. Epub 2020 Apr 23. 10.1016/j.ymben.2020.04.008 PubMed 32335188