SBC015869
(Plasmid
#226281)
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PurposeExpresses MsCAD from trc promoter; expresses BsSfp and SrCAR from a separate trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226281 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepBbE1c
- Total vector size (bp) 9212
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert name4'-phosphopantetheinyl transferase Sfp
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Alt namesfp
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SpeciesBacillus subtilis
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Insert Size (bp)678
Gene/Insert 2
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Gene/Insert nameCinnamyl alcohol dehydrogenase
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Alt nameCAD2
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SpeciesMedicago sativa
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Insert Size (bp)1077
Gene/Insert 3
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Gene/Insert nameCarboxylic acid reductase
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Alt nameCAR
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SpeciesSegniliparus rugosus
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Insert Size (bp)3567
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UniProt IDs:
CAD2 - P31656
CAR - E5XP76
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SBC015869 was a gift from Rainer Breitling (Addgene plasmid # 226281 ; http://n2t.net/addgene:226281 ; RRID:Addgene_226281) -
For your References section:
Carboxylic acid reductase-dependent biosynthesis of eugenol and related allylphenols. Hanko EKR, Valdehuesa KNG, Verhagen KJA, Chromy J, Stoney RA, Chua J, Yan C, Roubos JA, Schmitz J, Breitling R. Microb Cell Fact. 2023 Nov 18;22(1):238. doi: 10.1186/s12934-023-02246-4. 10.1186/s12934-023-02246-4 PubMed 37980525