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Addgene

SBC015866
(Plasmid #226280)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 226280 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    pBbE1c
  • Total vector size (bp) 8825
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    4'-phosphopantetheinyl transferase Sfp
  • Alt name
    sfp
  • Species
    Bacillus subtilis
  • Insert Size (bp)
    678

Gene/Insert 2

  • Gene/Insert name
    Cinnamyl alcohol dehydrogenase
  • Alt name
    CAD2
  • Species
    Medicago sativa
  • Insert Size (bp)
    1077

Gene/Insert 3

  • Gene/Insert name
    Carboxylic acid reductase
  • Alt name
    CAR
  • Species
    Segniliparus rugosus
  • Insert Size (bp)
    3567

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt IDs:
CAD2 - P31656
CAR - E5XP76

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SBC015866 was a gift from Rainer Breitling (Addgene plasmid # 226280 ; http://n2t.net/addgene:226280 ; RRID:Addgene_226280)
  • For your References section:

    Carboxylic acid reductase-dependent biosynthesis of eugenol and related allylphenols. Hanko EKR, Valdehuesa KNG, Verhagen KJA, Chromy J, Stoney RA, Chua J, Yan C, Roubos JA, Schmitz J, Breitling R. Microb Cell Fact. 2023 Nov 18;22(1):238. doi: 10.1186/s12934-023-02246-4. 10.1186/s12934-023-02246-4 PubMed 37980525