pRS316-SEC18-L1374
(Plasmid
#226275)
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PurposePlasmid expressing the SEC18 allele from L-1374, under control of its native promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226275 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
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Backbone manufacturerSikorski and Hieter, 1989, Genetics
- Backbone size w/o insert (bp) 4887
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSEC18
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Alt nameYBR080C
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSEC18 (a.k.a. YBR080C, ANU4)
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.08.28.610086 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS316-SEC18-L1374 was a gift from Jolanda Van Leeuwen (Addgene plasmid # 226275 ; http://n2t.net/addgene:226275 ; RRID:Addgene_226275) -
For your References section:
Genetic suppression interactions are highly conserved across genetic backgrounds. Paltenghi C, van Leeuwen J. bioRxiv 2024.08.28.610086 10.1101/2024.08.28.610086