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pRS315-SEC22-S288C
(Plasmid #226270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226270 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone manufacturer
    Sikorski and Hieter, 1989, Genetics
  • Backbone size w/o insert (bp) 6018
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEC22
  • Alt name
    YLR268W
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    SEC22 (a.k.a. YLR268W, SLY2, TS26, TSL26)

Cloning Information

  • Cloning method Other

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.28.610086 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS315-SEC22-S288C was a gift from Jolanda Van Leeuwen (Addgene plasmid # 226270 ; http://n2t.net/addgene:226270 ; RRID:Addgene_226270)

  • For your References section:

    Genetic suppression interactions are highly conserved across genetic backgrounds. Paltenghi C, van Leeuwen J. bioRxiv 2024.08.28.610086 10.1101/2024.08.28.610086
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