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pML104-LEU2-2
(Plasmid #226263)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226263 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pML104
  • Backbone manufacturer
    Laughery et al, 2015, Yeast
  • Backbone size w/o insert (bp) 11240
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LEU2 gRNA
  • Alt name
    YCL018W
  • gRNA/shRNA sequence
    GGTAGTGTTAGACCTGAACA
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    LEU2 (a.k.a. YCL018W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.28.610086 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pML104-LEU2-2 was a gift from Jolanda Van Leeuwen (Addgene plasmid # 226263 ; http://n2t.net/addgene:226263 ; RRID:Addgene_226263)

  • For your References section:

    Genetic suppression interactions are highly conserved across genetic backgrounds. Paltenghi C, van Leeuwen J. bioRxiv 2024.08.28.610086 10.1101/2024.08.28.610086
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