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pJB041
(Plasmid #22626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22626 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p18CAT
  • Backbone manufacturer
    R. McKinnon
  • Backbone size w/o insert (bp) 4728
  • Vector type
    CAT reporter plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human genomic PLP promoter region
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1088
  • Mutation
    Site 3 mutation -117 to -116bp GG to cc
  • Entrez Gene
    POU3F2 (a.k.a. BRN2, N-Oct3, OCT7, OTF-7, OTF7, POUF3, brn-2, oct-7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert is 1200bp (approx) of the Human PLP 5' upstream, promoter region and Exon1 (-1088 to +85) in the correct orientation. Site 3 (bp -117 to -116) were mutated via Site-Directed Mutagenesis from GG to cc.

This PLP DNA cloned by Hudson lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB041 was a gift from Lynn Hudson (Addgene plasmid # 22626 ; http://n2t.net/addgene:22626 ; RRID:Addgene_22626)

  • For your References section:

    Identification of cis-regulatory elements in the myelin proteolipid protein (PLP) gene. Berndt JA, Kim JG, Hudson LD. J Biol Chem. 1992 Jul 25. 267(21):14730-7. PubMed 1378839
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