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Addgene

pRSF_ApyADO
(Plasmid #226248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    His6_ApyA
  • Species
    Burkholderia thailandensis E264, codon optimized for E.coli expression
  • Tag / Fusion Protein
    • Hexa-Histidine (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    ApyD
  • Species
    Burkholderia thailandensis E264, codon optimized for E.coli expression

Gene/Insert 3

  • Gene/Insert name
    ApyO
  • Species
    Burkholderia thailandensis E264, codon optimized for E.coli expression

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF_ApyADO was a gift from Wilfred van der Donk (Addgene plasmid # 226248 ; http://n2t.net/addgene:226248 ; RRID:Addgene_226248)

  • For your References section:

    Biosynthesis of Macrocyclic Peptides with C-Terminal beta-Amino-alpha-keto Acid Groups by Three Different Metalloenzymes. Nguyen DT, Zhu L, Gray DL, Woods TJ, Padhi C, Flatt KM, Mitchell DA, van der Donk WA. ACS Cent Sci. 2024 Apr 11;10(5):1022-1032. doi: 10.1021/acscentsci.4c00088. eCollection 2024 May 22. 10.1021/acscentsci.4c00088 PubMed 38799663
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