pSCrhaB2_ApyOAHID
(Plasmid
#226245)
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PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSCrhaB2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Trimethoprim, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameHis6_ApyA
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SpeciesBurkholderia thailandensis E264
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Tag
/ Fusion Protein
- Hexa-Histidine (N terminal on insert)
Gene/Insert 2
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Gene/Insert nameApyD
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SpeciesBurkholderia thailandensis E264
Gene/Insert 3
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Gene/Insert nameApyO
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SpeciesBurkholderia thailandensis E264
Gene/Insert 4
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Gene/Insert nameApyH
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SpeciesBurkholderia thailandensis E264
Gene/Insert 5
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Gene/Insert nameApyI
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SpeciesBurkholderia thailandensis E264
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSCrhaB2_ApyOAHID was a gift from Wilfred van der Donk (Addgene plasmid # 226245 ; http://n2t.net/addgene:226245 ; RRID:Addgene_226245) -
For your References section:
Biosynthesis of Macrocyclic Peptides with C-Terminal beta-Amino-alpha-keto Acid Groups by Three Different Metalloenzymes. Nguyen DT, Zhu L, Gray DL, Woods TJ, Padhi C, Flatt KM, Mitchell DA, van der Donk WA. ACS Cent Sci. 2024 Apr 11;10(5):1022-1032. doi: 10.1021/acscentsci.4c00088. eCollection 2024 May 22. 10.1021/acscentsci.4c00088 PubMed 38799663