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Addgene

pCHC027 (∆pemK)
(Plasmid #226204)

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Item Catalog # Description Quantity Price (USD)
Plasmid 226204 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pK18msB
  • Backbone manufacturer
    Christopher Johnson, Addgene plasmid #177839
  • Vector type
    Synthetic Biology
  • Selectable markers
    KanR, SacB

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Upstream/downstream homology arms for addiction system toxinPemK.
  • Species
    Cupriavidus necator H16

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCHC027 (∆pemK) was a gift from Christopher Johnson (Addgene plasmid # 226204 ; http://n2t.net/addgene:226204 ; RRID:Addgene_226204)

  • For your References section:

    Improving growth of Cupriavidus necator H16 on formate using adaptive laboratory evolution-informed engineering. Calvey CH, Sanchez I Nogue V, White AM, Kneucker CM, Woodworth SP, Alt HM, Eckert CA, Johnson CW. Metab Eng. 2023 Jan;75:78-90. doi: 10.1016/j.ymben.2022.10.016. Epub 2022 Nov 9. 10.1016/j.ymben.2022.10.016 PubMed 36368470
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