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Addgene

pRSET.HaloTag.v7
(Plasmid #226197)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226197 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2869
  • Total vector size (bp) 3763
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag.v7
  • Insert Size (bp)
    894
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET.HaloTag.v7 was a gift from Tim Brown & HHMI-JRC Tool Translation Team (Addgene plasmid # 226197 ; http://n2t.net/addgene:226197 ; RRID:Addgene_226197)
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