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Addgene

pDIV270
(Plasmid #226181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226181 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDIV151
  • Total vector size (bp) 10950
  • Vector type
    Bacterial Expression, Yeast Expression, CRISPR
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tRNA-sgRNA-tRNA
  • Alt name
    sgRNA targeting KU70 locus in K.phaffii: AAGCAATACGACATCCACGA
  • Species
    Synthetic; Komagataella phaffii
  • Promoter TEF1p (Komagataella phaffii)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDIV270 was a gift from Uffe Mortensen (Addgene plasmid # 226181 ; http://n2t.net/addgene:226181 ; RRID:Addgene_226181)

  • For your References section:

    Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii. Strucko T, Gadar-Lopez AE, Frohling FB, Frost ET, Iversen EF, Olsson H, Jarczynska ZD, Mortensen UH. FEMS Yeast Res. 2024 Aug 23:foae026. doi: 10.1093/femsyr/foae026. 10.1093/femsyr/foae026 PubMed 39179418
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