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PiggyBac-mProser1-FLAG-IRES-Blast
(Plasmid #226173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226173 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac-GW-IRES-Blast
  • Backbone size w/o insert (bp) 7241
  • Total vector size (bp) 9980
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Proline Serine Rich protein 1
  • Alt name
    PROSER1
  • Alt name
    PRSR1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2739
  • Entrez Gene
    Proser1 (a.k.a. 2810046L04Rik, 9330161F11)
  • Tag / Fusion Protein
    • 2X FLAG (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.31.606086 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac-mProser1-FLAG-IRES-Blast was a gift from Kasper Rasmussen (Addgene plasmid # 226173 ; http://n2t.net/addgene:226173 ; RRID:Addgene_226173)

  • For your References section:

    PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations. Fleming A, Knatko EV, Li X, Zoch A, Heckhausen Z, Stransky S, Brenes AJ, Sidoli S, Hajkova P, O'Carroll D, Rasmussen KD. Genes Dev. 2024 Nov 19. doi: 10.1101/gad.352176.124. 10.1101/gad.352176.124 PubMed 39562138
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