PiggyBac-FLAG-mProser1-IRES-Blast
(Plasmid
#226172)
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PurposeExpresses full length mouse PROSER1 with 2X N-terminal FLAG tags
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac-GW-IRES-Blast
- Backbone size w/o insert (bp) 7241
- Total vector size (bp) 9980
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProline Serine Rich protein 1
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Alt namePROSER1
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Alt namePRSR1
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SpeciesM. musculus (mouse)
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Insert Size (bp)2739
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Entrez GeneProser1 (a.k.a. 2810046L04Rik, 9330161F11)
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Tag
/ Fusion Protein
- 2X FLAG (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.31.606086 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PiggyBac-FLAG-mProser1-IRES-Blast was a gift from Kasper Rasmussen (Addgene plasmid # 226172 ; http://n2t.net/addgene:226172 ; RRID:Addgene_226172) -
For your References section:
PROSER1 modulates DNA demethylation through dual mechanisms to prevent syndromic developmental malformations. Fleming A, Knatko EV, Li X, Zoch A, Heckhausen Z, Stransky S, Brenes AJ, Sidoli S, Hajkova P, O'Carroll D, Rasmussen KD. Genes Dev. 2024 Nov 19. doi: 10.1101/gad.352176.124. 10.1101/gad.352176.124 PubMed 39562138