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Addgene

CRaf-IRES-pErk reporter
(Plasmid #226170)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226170 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Total vector size (bp) 9632
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Myc-CRaf-IRES-3xFlag-tdMCP-Erk2
  • Alt name
    CRaf
  • Alt name
    Erk2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPK1 (a.k.a. ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK)
  • Entrez Gene
    RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • 3xFlag-tdMCP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRaf-IRES-pErk reporter was a gift from Dustin Maly (Addgene plasmid # 226170 ; http://n2t.net/addgene:226170 ; RRID:Addgene_226170)

  • For your References section:

    Multiplexed profiling of intracellular protein abundance, activity, interactions and druggability with LABEL-seq. Simon JJ, Fowler DM, Maly DJ. Nat Methods. 2024 Nov;21(11):2094-2106. doi: 10.1038/s41592-024-02456-7. Epub 2024 Oct 21. 10.1038/s41592-024-02456-7 PubMed 39433876
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