pJB044
(Plasmid
#22617)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep18CAT
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Backbone manufacturerR. McKinnon
- Backbone size w/o insert (bp) 4728
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Vector typeCAT reporter plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman genomic PLP promoter region
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SpeciesH. sapiens (human)
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Insert Size (bp)609
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Entrez GenePOU3F2 (a.k.a. BRN2, N-Oct3, OCT7, OTF-7, OTF7, POUF3, brn-2, oct-7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert is 609bp of the Human PLP 5' upstream, promoter region and Exon 1 (-524 to +85)
This PLP DNA cloned by Hudson lab.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJB044 was a gift from Lynn Hudson (Addgene plasmid # 22617 ; http://n2t.net/addgene:22617 ; RRID:Addgene_22617) -
For your References section:
Identification of cis-regulatory elements in the myelin proteolipid protein (PLP) gene. Berndt JA, Kim JG, Hudson LD. J Biol Chem. 1992 Jul 25. 267(21):14730-7. PubMed 1378839