TurboID-MEK1
(Plasmid
#226169)
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PurposeUsed to transiently express a TurboID fusion of the protein kinase MEK1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5
- Total vector size (bp) 7291
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMyc-TurboID-MEK1
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Alt nameMEK1
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SpeciesH. sapiens (human)
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Entrez GeneMAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
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Tag
/ Fusion Protein
- Myc-TurboID (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TurboID-MEK1 was a gift from Dustin Maly (Addgene plasmid # 226169 ; http://n2t.net/addgene:226169 ; RRID:Addgene_226169) -
For your References section:
Multiplexed profiling of intracellular protein abundance, activity, interactions and druggability with LABEL-seq. Simon JJ, Fowler DM, Maly DJ. Nat Methods. 2024 Nov;21(11):2094-2106. doi: 10.1038/s41592-024-02456-7. Epub 2024 Oct 21. 10.1038/s41592-024-02456-7 PubMed 39433876