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Addgene

(attB) tdMCP-tagged BRaf
(Plasmid #226165)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom attB
  • Total vector size (bp) 8326
  • Vector type
    Mammalian Expression ; Bxb1 attB, no promoter
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFlag-tdMCP-BRaf
  • Alt name
    BRaf
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Tags / Fusion Proteins
    • 3xFlag-tdMCP (N terminal on insert)
    • IRES-mCherry-P2A-PuroR (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (attB) tdMCP-tagged BRaf was a gift from Dustin Maly (Addgene plasmid # 226165 ; http://n2t.net/addgene:226165 ; RRID:Addgene_226165)

  • For your References section:

    Multiplexed profiling of intracellular protein abundance, activity, interactions and druggability with LABEL-seq. Simon JJ, Fowler DM, Maly DJ. Nat Methods. 2024 Nov;21(11):2094-2106. doi: 10.1038/s41592-024-02456-7. Epub 2024 Oct 21. 10.1038/s41592-024-02456-7 PubMed 39433876
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