RNA-Protein Landing Pad
(Plasmid #226162)

• Purpose: Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
• Depositing Lab: Dustin Maly
• Publication: Massively parallel profiling of the intracellular activity, interactions, and druggability of protein variants using LABEL-seq (unpublished)

Backbone

• Vector backbone: Custom
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Hygromycin ; AP1903

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mTagBFP2-P2A-iCasp9
• Species: Synthetic
• Insert Size (bp): 2025
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Reverse tetracycline transactivator 3
• Species: Synthetic
• Insert Size (bp): 705

Gene/Insert 3

• Gene/Insert name: Hygromycin-IRES-EGFP
• Insert Size (bp): 2418

Resource Information

• Supplemental Documents:
  • RNA-Protein Landing Pad.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry