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Addgene

RNA-Protein Landing Pad
(Plasmid #226162)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226162 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    Custom
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Hygromycin ; AP1903

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    mTagBFP2-P2A-iCasp9
  • Species
    Synthetic
  • Insert Size (bp)
    2025
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Reverse tetracycline transactivator 3
  • Species
    Synthetic
  • Insert Size (bp)
    705

Gene/Insert 3

  • Gene/Insert name
    Hygromycin-IRES-EGFP
  • Insert Size (bp)
    2418

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RNA-Protein Landing Pad was a gift from Dustin Maly (Addgene plasmid # 226162 ; http://n2t.net/addgene:226162 ; RRID:Addgene_226162)
  • For your References section:

    Multiplexed profiling of intracellular protein abundance, activity, interactions and druggability with LABEL-seq. Simon JJ, Fowler DM, Maly DJ. Nat Methods. 2024 Nov;21(11):2094-2106. doi: 10.1038/s41592-024-02456-7. Epub 2024 Oct 21. 10.1038/s41592-024-02456-7 PubMed 39433876